دیتیوبیس(اسید 2 نیتروبنزوئیک) کد D8130 سیگما

Synonym: 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

Description

General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent.^[2] It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH.^[1] Thiolate anion (RS^–) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^–). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.^[3]

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues.^[1] It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

Packaging

1, 5, 10, 25, 100 g in poly bottle

500 mg in glass insert