Carboxypeptidase B from porcine pancreas C9584 Sigma

Product Overview: Carboxypeptidase B Code C9584 Sigma-Aldrich

Carboxypeptidase B, supplied under product code C9584 by Sigma-Aldrich, is a high-grade proteolytic enzyme widely utilized in biochemical research, proteomics, and diagnostic assay development. Historically and systematically designated as Peptidyl-L-lysine(L-arginine) hydrolase or Protaminase under enzyme commission number EC 3.4.17.2, this exopeptidase specifically catalyzes the hydrolytic cleavage of basic amino acid residues, namely L-lysine and L-arginine, from the C-terminal position of peptides and proteins. Because of its stringent substrate specificity, Sigma Carboxypeptidase B serves as a vital biochemical tool for protein characterization, C-terminal sequence verification, and the targeted modification of cell-surface or soluble peptide domains.

Key Features and Advantages of Sigma Carboxypeptidase B Code C9584

High specificity toward C-terminal basic amino acids with minimal non-specific proteolysis

Exceptional enzymatic stability and batch-to-batch consistency under recommended assay protocols

Validated utility in homogeneous time-resolved fluorescence (HTRF) and high-throughput screening assays

Crucial biological reagent for biomarker discovery, diagnostic development, and microbial interaction studies

Produced under stringent Sigma-Aldrich quality standards to ensure maximal specific activity

Backed by complete analytical certification and guaranteed authenticity through Kian Parto Tadjhiz

Technical and Biochemical Specifications: Carboxypeptidase B Sigma C9584

Product Name: Carboxypeptidase B

Synonyms: Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase

Product Code: C9584

Manufacturer Brand: Sigma-Aldrich

CAS Number: 9025-24-5

Enzyme Commission Number: EC 3.4.17.2

Form: Lyophilized powder or buffered aqueous solution

Storage Conditions: Store at minus 20 degrees Celsius; avoid repeated freeze-thaw cycles

Target Applications: Protein sequence analysis, high-throughput fluorescence screening, acute pancreatitis biomarker evaluation, pregnancy biomarker assessment in wildlife, flow cytometry binding assays

Research and Methodological Applications

Carboxypeptidase B code C9584 has demonstrated diverse and impactful research applications. It has played an instrumental role in the development of non-invasive pregnancy monitoring assays for polar bears in both captive and wild environments. In clinical and medical research, the enzyme is employed in identifying and validating novel biomarker candidates for acute pancreatitis. Furthermore, this Sigma-Aldrich enzyme has been utilized to set up homogeneous time-resolved fluorescence (HTRF) formats to accurately monitor carboxypeptidase activity in high-throughput screening platforms. In microbiology and cellular immunology, Carboxypeptidase B treatment has been used to assess the role of C-terminal lysine residues in the binding of human plasminogen to Bifidobacterium species via flow cytometric analysis.

Why Order Sigma Carboxypeptidase B from Kian Parto Tadjhiz

Enzyme activity and structural integrity require optimal storage conditions and strict adherence to cold chain logistics. Kian Parto Tadjhiz guarantees the supply of authentic Sigma-Aldrich Carboxypeptidase B code C9584 with full documentation, rigorous quality control, and reliable temperature-controlled delivery to meet the demanding requirements of academic, clinical, and industrial research laboratories.