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گوانیدین هیدروکلراید

Synonym: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

Description

Application

Guanidine hydrochloride has been used:
• as a component of the extraction buffer for the extraction of proteoglycans
• in extraction during protein fractionation of ATDC5 cell lines
• as a chemical additive to study its effective absorbance spectra in structural analysis[1]
• to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer[12]
• as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study[9]
• in RNA isolation to dissociate nucleoproteins and inhibit RNase

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Packaging

1, 2 kg in poly bottle

100, 500 g in poly bottle

Biochem/physiol Actions

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule.[13][2] It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo.[13] It is a potent denaturant and inactivator of several enzymes and proteins.[2][3] GuHCl can inactivate aminoacylase and papain.[3] It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor.[3] At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Properties

Related Categories Biochemicals, Biochemicals and Reagents, Buffers / Buffer Salts, Chaotropic Agents, Core Bioreagents,

Quality Level 400

biological source synthetic (organic)

grade for molecular biology

assay ≥99%

form crystalline powder

application(s) RNA extraction: suitable

impurities ≤0.3% water (Karl Fischer)

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