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Synonym: Endopeptidase K

Description

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.

Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.

Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria

Determination of enzyme localization on membranes

Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.

Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Packaging

1 g in poly bottle

10, 25, 100, 500 mg in poly bottle

5 mg in glass bottle

Properties

Quality Level 400

grade for molecular biology

product line BioUltra

form lyophilized powder

mol wt 28.93 kDa

composition Protein, ≥90% biuret

impurities ≤1 ppm DNA

foreign activity DNAse, Nickase and RNAse, none detected

shipped in wet ice

storage temp. −20°C

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