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Synonym: 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

Description

General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent.[2] It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH.[1] Thiolate anion (RS) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.[3]

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues.[1] It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

Packaging

1, 5, 10, 25, 100 g in poly bottle

500 mg in glass insert

Properties

Related Categories Amino Acid Analysis, Amino Acid Analysis Reagents, Analytical and Industrial Enzymes, Biochemicals and Reagents, Chemical Synthesis,

Quality Level 200

product line BioReagent

assay ≥98%

mp 240-245 °C (dec.) (lit.)

suitability suitable for determination of sulfhydryl groups

greener alternative category Re-engineered

SMILES string OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

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